LES Phage 5 length, capsidated, amplification, and homologous with other Pseudomonas phages.
Reem A. Talat 1,2*, Enrique González-Tortuero 3,4, Revathy Krishnamurthi 1, Jo L. Fothergill 1, Chloe E. James 3, Heather E. Allison 1
- Institute of Infection, Veterinary and Ecological Sciences (IVES), University of Liverpool, L69 7ZB. United Kingdom.
- College of Environment sciences and Technology, University of Mosul. Iraq.
- School of Science, Engineering and Environment (SEE), University of Salford, M5 4WT. United Kingdom.
- European Virus Bioinformatics Center, Leutragraben 1, 07743 Jena, Germany.
P. aeruginosa LES is an opportunistic transmissible pathogen between CF patients, being capable of infecting the lung of non-CF patients and superinfecting patients with other P. aeruginosa strains. Inside P. aeruginosa LES genome, 20 genomic islands were which included the presence of five active prophages and one prophage-like element. LES φ5 is a temperate and active Pseudomonas prophage that plays an essential role in host's competitiveness. Seventy-six ORFs are predicted in LES Pseudomonas phage 5. Only 20 ORFs do not have functional prediction (Hypothetical protein). We proved that the total length of LES phage 5 genome is 50,235 bp, and the attL and attR regions were located at 2,690,327 – 2,690,341 and 2,740,547 – 2,740,561 in the P. aeruginosa LESB58 genome, respectively. It was proved that LES phage 5 is complete phage because it encapsidated its DNA.
There are 3 phages share homologous sequences with LES phage 5. Moreover, LES phage 2,3,4 and 5 have spontaneous induction in the log and stationery phase. Differences between the amplification of all LES phages were detected.