Without action, antibiotic resistance could result in the death of 10 million people by 2050. Fortunately, phage therapy offers an alternative solution that uses bacteriophages or phages to fight bacteria. However, only phages with specific characteristics are suitable for therapy. They must be efficient, toxin-free, and lacking virulence factors. Isolating these specific phages can be challenging and labor-intensive. Yet, we have developed a targeted phage isolation method that enables the screening of suitable phages from both environmental and human-derived samples. In addition, we can evaluate their efficacy on both the population and single-cell levels. By using this method, we have also discovered significant cell-to-cell heterogenicity that varies depending on the type of phage tested. Going forward, it would be interesting to evaluate the effect of this variation on the therapeutic potential of phages.